DNA Extraction and Purification
Zymo Research Corporation Human Methylated & Non-Methylated (WGA) DNA Set (DNA w/ primers)
The Human WGA Methylated & Non-methylated DNA Set isgenerated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CGdinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.
Zymo Research Corporation ZymoBIOMICS™ DNA Binding Buffer, 250 mL
It is certified to be low-bioburden to ensure minimal background contaminants for low abundance microbiome samples.
Zymo Research Corporation ZymoBIOMICS HRC Prep Solution, 30 ml
It is certified to be low-bioburden to ensure minimal background contaminants for low abundance microbiome samples.
Zymo Research Corporation M-Binding Buffer, 250 ml
Used for purification of bisulfite converted DNA on a Zymo-spin column in the EZ DNA Methylation, EZ DNA Methylation - Gold, EZ DNA Methylation - Direct kits, and EZ DNA Methylation - Lightning kits.
Zymo Research Corporation ZymoPURE™ P2 (Green) (13 ml)
ZymoPURE P2 (Blue) is designed to be used with our ZymoPURE Plasmid Purification Kits, which is the fastest and simplest method available to efficiently isolate transfection-grade plasmid DNA from E. coli.
Zymo Research Corporation ZymoPURE™ Wash 1 (320 ml)
ZymoPURE Wash 1 is designed to be used with our ZymoPURE Plasmid Purification Kits, which is the fastest and simplest method available to efficiently isolate transfection-grade plasmid DNA from E. coli.
LAMDA BIOTECH INC Column-Pure™ PCR Clean-Up Kit
This kit is designed for purification of PCR products ranging from 100bp to 10kb. Salt, primers, enzymes, dNTPs and other impurities will be removed from the PCR reaction products. The incorporation of a new technology also allows the kit to be used to concentrate DNA by eluting samples in small volumes.
Epigentek Inc MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric) (96 assays)
For absorbance-based quantitation of global DNA hydroxymethylation in an ELISA-like format
Epigentek Inc EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (Colorimetric) (48 assays)
For quantitating 8OHdG for oxidative DNA damage in DNA samples
New England Biolabs, Inc. NEBNext® Ultra™ II Q5® Master Mix – 500 reactions
The NEBNext Ultra II Q5 Master Mix is the most recent formulation of Q5 DNA polymerase that has been optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries. This new formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions. The polymerase component of the master mix, Q5 High-Fidelity DNA Polymerase, is a novel thermostable DNA polymerase that possesses 3´- 5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5 also has the highest fidelity available (> 280-fold higher than that of Taq DNA Polymerase), resulting in ultra-low error rates. The NEBNext Ultra II Q5 Master Mix is an aptamer-based hot start formulation that allows simple room temperature reaction set up. The convenient 2X master mix format contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification.
Biotium AccuGreen™ Broad Range dsDNA Quantitation Solution (500 assays)
The AccuGreen™ Broad Range dsDNA Quantitation Solution is designed for use with handheld fluorometers such as the Qubit fluorometer from Thermo Fisher. The AccuGreen™ quantitation solution is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL. This item is a standalone fluorescent DNA quantitation solution. The use of this product on the Qubit fluorometer requires two standards. The assay has a green fluorescence with an excitation/emission of 500/530 nm (with DNA). This product has sufficient reagent for 500 assays.